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Venom components from Citharischius crawshayi spider

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  • Venom components from Citharischius crawshayi spider

    Diego-GarcĂ­a, E., S. Peigneur, E. Waelkens, S. Debaveye & J. Tytgat. 2010. Venom components from Citharischius crawshayi spider (Family Theraphosidae): exploring transcriptome, venomics, and function. Cellular and Molecular Life Sciences Published online: 6 April 2010

    Abstract. Despite strong efforts, knowledge about the composition of the venom of many spider species remains very limited. This work is the first report of transcriptome and venom analysis of the African spider Citharischius crawshayi. We used combined protocols of transcriptomics, venomics, and biological assays to characterize the venom and genes expressed in venom glands. A cDNA library of the venom glands was constructed and used to generate expressed sequence tags (ESTs). Sequence comparisons from 236 ESTs revealed interesting and unique sequences, corresponding to toxin-like and other components. Mass spectrometrical analysis of venom fractions showed more than 600 molecular masses, some of which showed toxic activity on crickets and modulated sodium currents in DmNav1 and Nav1.6 channels as expressed in Xenopus oocytes. Taken together, our results may contribute to a better understanding of the cellular processes involved in the transcriptome and help us to discover new components from spider venom glands with therapeutic potential.

    Spider Myths | Curious Taxonomy | The World Spider Catalog - Theraphosidae

    "We are all taxonomists." -Judith Winston
    "The laws of biology are written in the language of diversity." -Edward Osborne Wilson
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